Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet Engineering
نویسندگان
چکیده
منابع مشابه
Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering
In the past two decades, as a novel approach for tissue engineering, cell sheet engineering has been proposed by our laboratory. Poly(N-isopropylacrylamide) (PIPAAm), which is a well-known temperature-responsive polymer, has been grafted on tissue culture polystyrene (TCPS) surfaces through an electron beam irradiated polymerization. At 37 °C, where the PIPAAm modified surface is hydrophobic, c...
متن کاملRecent development of temperature-responsive surfaces and their application for cell sheet engineering.
Cell sheet engineering, which fabricates sheet-like tissues without biodegradable scaffolds, has been proposed as a novel approach for tissue engineering. Cells have been cultured and proliferate to confluence on a temperature-responsive cell culture surface at 37°C. By decreasing temperature to 20°C, an intact cell sheet can be harvested from the culture surface without enzymatic treatment. Th...
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background : in recent years tissue engineering developed to replace or repair damaged tissues using cell and biomaterial. one of the most important fields of tissue engineering is simulation of in vivo micro environment of body tissues. this study aimed to develop culture chamber and cell biomaterial sheet engineering for the reconstruction of cartilage tissue. methods : stainless steel cultur...
متن کاملTemperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering.
Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly(N-isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In th...
متن کاملSurface modification of uniaxial cyclic strain cell culture platform with temperature-responsive polymer for cell sheet detachment.
Current cell sheet-based blood vessels lack biomimetic structure and require excessively long culture times that may compromise smooth muscle cell phenotype. We modified a commercially available product for uniaxial cell sheet conditioning with thermoresponsive copolymers. Thus, culture of detachable conditioned cell sheets is shortened while retaining structural integrity and contractility.
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ژورنال
عنوان ژورنال: Cyborg and Bionic Systems
سال: 2021
ISSN: 2692-7632
DOI: 10.34133/2021/5738457